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Main PlantSeg Workflow

Widget: Neural Network Prediction - Find Boundary

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  • Mode: Select the mode to run the prediction.
  • Image: Raw image to be processed with a neural network.
  • Model filter: Choose to only show models tagged with `plantseg`.
  • PlantSeg model: Select a pretrained PlantSeg model. Current model description: Unet trained on confocal images of Arabidopsis Ovules on 1/2-resolution in XY with BCEDiceLoss.
  • BioImage.IO model: Select a model from BioImage.IO model zoo.

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  • Mode: Select the mode to run the prediction.
  • Image: Raw image to be processed with a neural network.
  • Model filter: Choose to only show models tagged with `plantseg`.
  • BioImage.IO model: Select a model from BioImage.IO model zoo.

Widget: Watershed - Generate Superpixels

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  • Boundary image: Raw boundary image or boundary prediction to use as input for Watershed.
  • Mode: Define if the Watershed will run slice by slice (faster) or on the full volume (slower).
  • Boundary threshold: A low value will increase over-segmentation tendency and a large value increase under-segmentation tendency.
  • Minimum segment size: Minimum segment size allowed in voxels.
  • Show advanced parameters: Show advanced parameters for the Watershed algorithm.

Widget: Agglomeration - Merge Superpixels into Instances

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  • Boundary image: Raw boundary image or boundary prediction to use as input for clustering.
  • Nuclei foreground: Nuclei foreground prediction or segmentation.
  • Over-segmentation: Over-segmentation labels layer (superpixels) to use as input for clustering.
  • Agglomeration mode: Select which agglomeration algorithm to use.
  • Under/Over segmentation factor: A low value will increase under-segmentation tendency and a large value increase over-segmentation tendency.
  • Minimum segment size: Minimum segment size allowed in voxels.